NEPHELOstar Plus Microplate Nephelometer from BMG LABTECH

Product Specs
ItemNEPHELOstar Plus Microplate Nephelometer
CompanyBMG LABTECH
Price Get Pricing Get Quote for this Product
Catalog NumberNEPHELOstar Plus
QuantityEA
RangeUp to 45°C
Wellsup to 384
ApplicationsNephelometry (Light scattering)
Wavelength(s)635 ± 10 nm
Optical SystemDepends on particle size and liquid properties
Silica detection (particle size 0.5 to 10 µm) 800 nM

Dynamic Range: 5 decades
Maximum count rate (2,000,000 Relative Nephelometry Units (RNUs) per second)
Plate FormatsUp to 384-well plates

Specifications/Features

Shaking, Temperature Control, Injector

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BMG LABTECH

BMG LABTECH Allmendgruen 8

Ortenberg 77799

Germany

Phone: +49 781 96968-0

Email: sales@bmglabtech.com

Company Profile

Website: http://www.bmglabtech.com

(1) Optimization of a small molecule inhibitor of secondary nucleation in α-synuclein aggregationFrontiers in Molecular BiosciencesAugust 28, 2023Roxine Staats, Z. Faidon Brotzakis, Sean Chia, Robert I. Horne, Michele Vendruscoloa transparent cover. The change in scattering due to α-synuclein aggregation was monitored using a Nephelostar Plus (BMG Labtech, Aylesbury, United Kingdom) under quiescent conditions, using a laser intensity setting

(2) Cryo-EM Structure of a Human LECT2 Amyloid Fibril Reveals a Network of Polar Ladders at its CorebioRxivJuly 18, 2023Logan S. Richards, Maria D. Flores, Samantha Zink, Natalie A. Schibrowsky, Michael R. Sawaya, Jose A. Rodriguezpipetted in triplicate into a 96 well tray alongside buffer control wells for each and placed on a NEPHELOstar Plus plate reader (BMG Labtech). The tray was stirred every ten minutes to homogenize the mixture and light

(3) A new cheese population in Penicillium roqueforti and adaptation of the five populations to their ecological nicheEvolutionary ApplicationsJuly 10, 2023Ewen Crequer, Jeanne Ropars, Jean‐Luc Jany, Thibault Caron, Monika Coton, Alodie Snirc, Jean‐Philippe Vernadet, Antoine Branca, Tatiana Giraud, Emmanuel Cotondistilled water to regulate humidity during incubation. Measures were obtained using a laser nephelometer (NEPHELOstarPLUS, BMG labtech) with a laser diode at 635 nm. At the beginning of each measurement, a 20‐s 500 rpm double‐orbital

(4) Synthesis and Characterization of Phenylalanine Amides Active against Mycobacterium abscessus and Other MycobacteriaJournal of Medicinal ChemistryApril 13, 2023Markus Lang, Uday S. Ganapathy, Lea Mann, Rana Abdelaziz, Rüdiger W. Seidel, Richard Goddard, Ilaria Sequenzia, Sophie Hoenke, Philipp Schulze, Wassihun Wedajo Aragaw, et al.solubility screen The solubilities of the final compounds were determined nephelometrically using a NEPHELOstar Plus (BMG Labtech GmbH, GER) device. 25 mM stock solutions in DMSO of the compounds were prediluted in pure

(5) Green Drug Discovery: Novel Fragment Space from the Biomass-Derived Molecule Dihydrolevoglucosenone (CyreneTM)MoleculesFebruary 13, 2023Tom Dekker, Jaap W. Harteveld, Gábor Wágner, Max C. M. de Vries, Hans Custers, Andrea C. van de Stolpe, Iwan J. P. de Esch, Maikel WijtmansCyrene (Figure 1). 4. Materials and Methods 4.1. Nephelometry Nephelometry was performed using NEPHELOstar Plus equipment (BMG LABTECH, Ortenberg, Germany). Kaolin was used as the internal standard, and compounds

(6) Exploring the Effect of Halogenation in a Series of Potent and Selective A2B Adenosine Receptor AntagonistsJournal of Medicinal ChemistryJanuary 12, 2023Rubén Prieto-Díaz, Manuel González-Gómez, Hugo Fojo-Carballo, Jhonny Azuaje, Abdelaziz El Maatougui, Maria Majellaro, María I. Loza, José Brea, Víctor Fernández-Dueñas, M. Rita Paleo, et al.PBS (pH = 7). The plate was incubated at 37 °C for 4 h, and the light scattering was measured in a NEPHELOstar Plus reader (BMG LABTECH). The data was fitted to a segmented linear regression for measuring the compound

(7) De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystalsNature CommunicationsDecember 1, 2022Guillaume Tetreau, Michael R. Sawaya, Elke De Zitter, Elena A. Andreeva, Anne-Sophie Banneville, Natalie A. Schibrowsky, Nicolas Coquelle, Aaron S. Brewster, Marie Luise GrÃ¼nbein, Gabriela Nass Kovacs, et al.resuspending the crystal pellet in 150 µL ultrapure H2O and placed in a 96-well plate to be read on a NEPHELOstar Plus (BMG Labtech) nephelometer. These counts were normalized by subtracting the background signal and conducted

(8) From Serendipity to Rational Identification of the 5,6,7,8-Tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-4(3H)-one Core as a New Chemotype of AKT1 Inhibitors for Acute Myeloid LeukemiaPharmaceuticsOctober 26, 2022Andrea Astolfi, Francesca Milano, Deborah Palazzotti, Jose Brea, Maria Chiara Pismataro, Mariangela Morlando, Oriana Tabarrini, Maria Isabel Loza, Serena Massari, Maria Paola Martelli, et al.0.004%, MgCl2 10 mM, at a pH = 7.5. Incubation occurred at 37 °C and the results were read after 2 h in a NEPHELOstar Plus (BMG LABTECH, Ortenberg, Germany). The results were adjusted to a segmented regression to obtain the

(9) Periostin Augments Vascular Smooth Muscle Cell Calcification via β-Catenin SignalingBiomoleculesAugust 21, 2022Alesutan I, Henze LA, Boehme B, Luong TTD, Zickler D, Pieske B, Eckardt KU, Pasch A, Voelkl J.transition time (T50) of in vitro transformation from primary to secondary calciprotein particles using a Nephelostar Plus nephelometer (BMG Labtech, Ortenberg, Germany) was previously described in detail [34]. The serum periostin

(10) Allosteric modulation of dopamine D2L receptor in complex with Gi1 and Gi2 proteins: the effect of subtle structural and stereochemical ligand modificationsPharmacological ReportsApril 1, 2022Justyna Żuk, Damian Bartuzi, Andrea G. Silva, Monika Pitucha, Oliwia Koszła, Tomasz M. Wróbel, Dariusz Matosiuk, Marián Castro, Agnieszka A. Kaczorconcentration was excluded from the assays due to solubility problems as assessed by nephelometry (NEPHELOstar Plus instrument, BMG LABTECH GmbH, Ortenberg, Germany), see Fig. S14. Haloperidol (Sigma-Aldrich) (0.01 nM–1

(11) Optimization of 2-Amino-4,6-diarylpyrimidine-5-carbonitriles as Potent and Selective A₁ AntagonistsJournal of Medicinal ChemistryFebruary 10, 2022Cristina Val et al.(Greiner 781801) with 1% DMSO:99% PBS buffer. These were incubated at 37 °C and read after 2 h in a NEPHELOstar Plus (BMG LABTECH). The results were adjusted to a segmented regression to obtain the maximum concentration

(12) A 3D-Printed Polymer–Lipid-Hybrid Tablet towards the Development of Bespoke SMEDDS FormulationsPharmaceuticsDecember 7, 2021Bryce W. Barber, Camille Dumont, Philippe Caisse, George P. Simon, Ben J. Boydthe dispersion into the well of a 96-well UV-transparent microplate. Turbidity was determined using a Nephelostar Plus nephelometer (BMG Labtech) set to 37 °C, with a laser beam focus of 2.5 mm and laser intensity of 80%.

(13) From the Design to the In Vivo Evaluation of Benzohomoadamantane-Derived Soluble Epoxide Hydrolase Inhibitors for the Treatment of Acute PancreatitisJournal of Medicinal ChemistryMay 13, 2021Sandra Codony et al.containing 99 μL of PBS. The plate was incubated at 37 °C for 2 h and the light scattering was measured in a Nephelostar Plus reader (BMG LABTECH). The data were fitted to a segmented linear regression for measuring the compound

(14) 7-(2-Anilinopyrimidin-4-yl)-1-benzazepin-2-ones Designed by a “Cut and Glue” Strategy Are Dual Aurora A/VEGF-R Kinase InhibitorsMoleculesMarch 14, 2021Mehmet Karatas, Apirat Chaikuad, Bianca Berger, Michael H. G. Kubbutat, Frank Totzke, Stefan Knapp, Conrad Kunick(2:198) and measured as blank values. The 96 well plate prepared in this way was measured with the NEPHELOstar® Plus nephelometer (BMG Labtech GmbH, Ortenberg, Germany, software: Omega 5.11) at a temperature of 25 ◦ C.

(15) Synthesis and characterization of 1,2,4-triazolo[1,5-a]pyrimidine-2-carboxamide-based compounds targeting the PA-PB1 interface of influenza A virus polymeraseEuropean Journal of Medicinal ChemistryJanuary 1, 2021Serena Massari, Chiara Bertagnin, Maria Chiara Pismataro, Anna Donnadio, Giulio Nannetti, Tommaso Felicetti, Stefano Di Bona, Maria Giulia Nizi, Leonardo Tensi, Giuseppe Manfroni, et al.transparent plate (Greiner 781,801) with 1% DMSO: 99% PBS buffer, incubated at 37 °C and read after 2 h in a NEPHELOstar Plus (BMG LABTECH). The results were adjusted to a segmented regression to obtain the maximum concentration

(16) HSF1 physically neutralizes amyloid oligomers to empower overgrowth and bestow neuroprotectionScience AdvancesNovember 13, 2020Zijian Tang, Kuo-Hui Su, Meng Xu, Chengkai Daiwith 600 rpm shaking for 48 hours, and the turbidity was measured at different time points using a NEPHELOstar Plus microplate nephelometer (BMG Labtech). Transmission electron microscopy Following incubation, AFs or aggregates were pelleted

(17) 2-Oxaadamant-1-yl Ureas as Soluble Epoxide Hydrolase Inhibitors: In Vivo Evaluation in a Murine Model of Acute PancreatitisJournal of Medicinal ChemistrySeptember 10, 2020Sandra Codony et al.buffer. Then, they were incubated at 37 °C and the light scattering was measured after 2 hours in a NEPHELOstar Plus (BMG LABTECH). The results were adjusted to a segmented regression to obtain the maximum concentration

(18) Association between Serum Zinc and Calcification Propensity (T50) in Patients with Type 2 Diabetes Mellitus and In Vitro Effect of Exogenous Zinc on T50BiomedicinesSeptember 9, 2020Shinya Nakatani, Katsuhito Mori, Mika Sonoda, Kozo Nishide, Hideki Uedono, Akihiro Tsuda, Masanori Emoto, Tetsuo Shojiparticipants and/or by reviewing their medical records. 2.5. Devices, Plastic Materials, and Chemicals The Nephelostar Plus microplate reader with 2 built-in Reagent injectors and MARS software was obtained from BMG Labtech

(19) Cryo-EM structure of a human prion fibril with a hydrophobic, protease-resistant coreNature Structural & Molecular BiologyMay 1, 2020Calina Glynn, Michael R. Sawaya, Peng Ge, Marcus Gallagher-Jones, Connor W. Short, Ronquiajah Bowman, Marcin Apostol, Z. Hong Zhou, David S. Eisenberg, Jose A. Rodriguezof Proteinase K, growth buffer only, or water were added to a sealed 96-well plate and loaded into a NEPHELOstar Plus (BMG Labtech). Readings in nephelometry units (NTUs) were recorded at 37 °C for 0.1 seconds every 10

(20) Advancing Therapeutic Protein Discovery and Development through Comprehensive Computational and Biophysical CharacterizationMolecular PharmaceuticsJanuary 7, 2020Lorenzo Gentiluomo et al.was performed using a liquid handling system (Freedom-EVO 150, Tecan, Germany). Turbidity was measured using a NEPHELOstar Plus plate reader (BMG Labtech, Germany) after an incubation time of 48 hours. Non-linear regression analysis

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